Related Experiment Videos
Isolation and properties of Enterococcus hirae mutants defective in the potassium/proton antiport system
1Faculty of Pharmaceutical Sciences, Chiba University, Inage-ku, Chiba 263-8522, Japan. yoshimi@athenaeum.p.chiba-u.ac.jp
Journal of Bacteriology
|June 29, 1999
Abstract:
A K+/H+ antiporter regulates cytoplasmic pH in Enterococcus hirae growing at alkaline pH. Mutants defective in this antiport activity were alkaline pH sensitive. One mutant, Pop1, lacked both K+/methylamine exchange at pH 9.5 and concomitant acidification of cytoplasmic pH. Pop1 grew well at pHs below 8 but did not at pHs above 9, conditions under which cytoplasmic pH was not fully acidified.