Related Experiment Video
Updated: Jul 28, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Molecular epidemiological study of a mass outbreak caused by enteropathogenic Escherichia coli O157:H45
S Makino1, H Asakura, T Shirahata
1Department of Veterinary Microbiology, University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan. smakino@obihiro.ac.jp
Abstract:
We made a molecular analysis of O157:H45 Escherichia coli isolated from a mass outbreak that occurred in Obihiro City. Using DNA analysis, we confirmed this infection case as a mass outbreak. Although the isolates expressed O157 antigen, they did not produce Vero toxin. We concluded they were enteropathogenic E. coli (EPEC) because they had a bfp gene and an EAF plasmid, and further they exhibited local adherence to HEp-2 cells. We believe this is the first report of a mass outbreak by O157 EPEC, and we suggest that PCR using eae- and bfp-specific primers and HEp-2 adherence assay are useful to identify EPEC.
More Related Videos
06:20Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
Published on: April 9, 2018
09:44Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
Related Concept Videos
Investigation of Disease Outbreaks
Bacterial Gastroenteritis
Cholera