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Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

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Growth hormone (GH) and prolactin receptors in human peripheral blood mononuclear cells: relation with age and

J L Bresson¹, S Jeay, M C Gagnerault

¹INSERM Unité 344, Molecular Endocrinology, Centre d'Investigation Clinique, Hôpital Necker, Paris, France.

|June 29, 1999

Summary

Human growth hormone (GH) and prolactin (PRL) receptor expression in peripheral blood mononuclear cells (PBMC) varies with age. GH receptor (GHR) levels decrease in adults, while PRL receptor (PRLR) levels increase in younger adults compared to older adults.

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Area of Science:

Immunology
Endocrinology
Cell Biology

Background:

Growth hormone receptors (GHRs) and prolactin receptors (PRLRs) are crucial for mediating the effects of growth hormone (GH) and prolactin (PRL).
Understanding the expression patterns of these receptors in peripheral blood mononuclear cells (PBMC) is important for insights into immune function and endocrine regulation.
Previous studies have not fully elucidated the age- and sex-related variations in GHR and PRLR expression on human PBMC subsets.

Purpose of the Study:

To investigate the expression levels of GHR and PRLR in human PBMC.
To examine how GHR and PRLR expression varies across different age groups and in relation to sex.
To explore the correlation between plasma GH binding protein (GHBP) and GH receptor expression on PBMC.

Main Methods:

Flow cytometry was employed to quantify GHR and PRLR expression on human PBMC.
Biotinylated anti-GHR monoclonal antibody 10B8 and biotinylated human PRL were used for receptor detection.
Double immunofluorescence staining allowed for the analysis of receptor expression on specific PBMC subsets (T cells, B cells, monocytes).

Main Results:

Approximately 30% of total PBMC expressed GH receptors, with low expression on T cells and high expression on B cells and monocytes.
The percentage of PBMC expressing GH receptors was significantly lower in the 20-40 year age group compared to younger (12-20 yr) and older (>60 yr) groups.
PRLR levels were significantly higher in the 20-40 year age group compared to the >60 year group, and a negative correlation was found between plasma GHBP and PBMC GHR percentage.

Conclusions:

GHR and PRLR expression on human PBMC exhibits significant age-dependent variations.
The regulation of GH receptors may differ between lymphocytes and other target cells.
These findings highlight the dynamic nature of hormone receptor expression in immune cells throughout the lifespan.