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Related Experiment Videos

Asymmetric and node-specific nodal expression patterns are controlled by two distinct cis-acting regulatory elements.

D P Norris1, E J Robertson

  • 1Department of Molecular and Cellular Biology, Harvard University, The Biological Laboratories, Cambridge, Massachusetts 02138, USA.

Genes & Development
|July 1, 1999
PubMed
Summary

Nodal signaling establishes body axes in vertebrate embryos. A specific intronic enhancer controls Nodal expression in the epiblast, endoderm, and lateral plate mesoderm, crucial for body axis formation.

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Area of Science:

  • Developmental biology
  • Molecular genetics
  • Embryogenesis

Background:

  • Nodal, a TGFbeta-related molecule, is essential for vertebrate embryonic development.
  • Nodal regulates anterior-posterior (A-P) and left-right (L-R) body axis formation.
  • Nodal expression is dynamic and occurs at specific sites during embryogenesis.

Purpose of the Study:

  • To identify and analyze the cis-regulatory elements controlling Nodal expression domains during early development.
  • To understand how Nodal expression is regulated in different embryonic tissues and axes determination.

Main Methods:

  • Analysis of cis-regulatory elements controlling Nodal expression.
  • Targeted deletion of specific DNA regions within the Nodal locus.
  • Examination of Nodal expression patterns in the developing mouse embryo.

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Main Results:

  • Regulatory sequences for node-specific Nodal expression are located upstream.
  • A 600-bp intronic enhancer controls early endoderm/epiblast expression and asymmetric left-sided expression.
  • Deletion of a 100-bp subregion of the intronic enhancer disrupts Nodal expression in key developmental processes.

Conclusions:

  • A single intronic enhancer plays a critical role in regulating Nodal expression for both A-P and L-R axis formation.
  • Developmentally regulated Nodal expression at distinct sites may be controlled by common transcriptional activators.