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Effect of ultrasonic cleaning on microorganisms
M D Bettner1, M A Beiswanger, C H Miller
1Indiana University School of Dentistry, Indianapolis 46202, USA.
American Journal of Dentistry
|July 1, 1999
Summary
Ultrasonic cleaning effectively kills Streptococcus mutans at 60°C, with complete microbial elimination achieved in saline or detergent solutions. Bacterial escape into the air was negligible when the ultrasonic unit lid was used.
Area of Science:
- Microbiology
- Biotechnology
- Biochemistry
Background:
- Ultrasonic cleaning is widely used for disinfection.
- Assessing microbial kill efficacy and potential aerosolization is crucial for safety and effectiveness.
Purpose of the Study:
- To develop a method for quantifying microbial inactivation by ultrasonic cleaning.
- To evaluate bacterial escape from ultrasonic cleaning solutions.
Main Methods:
- Streptococcus mutans (S. mutans) suspensions were treated with enzymatic detergents or saline at 21°C, 37°C, or 60°C.
- Ultrasonic cleaning was applied for 20 minutes, with microbial sampling before and after.
- Control experiments assessed kill without ultrasound or detergent, and with detergent alone.
Main Results:
- Little kill (5-15%) occurred at 21°C or 37°C with detergents; 25-35% kill was observed with saline.
- Complete microbial kill was achieved at 60°C in saline or detergent solutions, with or without ultrasound.
- Bacterial escape was minimal and reduced to zero with the unit lid.
Conclusions:
- Sixty degrees Celsius is essential for effective ultrasonic cleaning of S. mutans.
- Ultrasonic cleaning efficacy is temperature-dependent.
- The use of a lid effectively contains potential microbial aerosols.