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Related Experiment Videos

HIV and SIV gp120 binding does not predict coreceptor function.

S S Baik1, R W Doms, B J Doranz

  • 1Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, 19104, USA.

Virology
|July 2, 1999
PubMed
Summary
This summary is machine-generated.

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Viral entry depends on HIV/SIV Envelope (Env) proteins binding coreceptors. This study found that gp120 binding doesn't always predict coreceptor function in viral fusion, unlike chemokine binding.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • HIV and SIV Envelope (Env) proteins mediate viral entry by interacting with cellular coreceptors.
  • Understanding these interactions is crucial for developing antiviral therapies.

Purpose of the Study:

  • To investigate the relationship between gp120 binding affinity and coreceptor function in viral entry.
  • To compare gp120 binding with chemokine binding to CCR5 mutants.

Main Methods:

  • Utilized a sensitive and specific gp120 binding assay.
  • Employed viral fusion assays with various HIV and SIV strains.
  • Analyzed CCR5 chimeras and mutants to assess binding and signaling.

Main Results:

Related Experiment Videos

  • Discordance observed between gp120 binding and Env-mediated membrane fusion.
  • HIV-1 strain 89.6 gp120 showed no detectable binding to its functional coreceptors.
  • gp120 from R5 strains and SIV showed weak or undetectable binding to functional CCR5 mutants.
  • Chemokine binding to CCR5 mutants accurately predicted their signaling ability.

    • Conclusions:

    • gp120 binding is more sensitive to CCR5 structural changes than chemokine binding.
    • Chemokine binding to CCR5 is a reliable predictor of coreceptor function.
    • gp120 binding is not always a reliable predictor of coreceptor function in viral entry.