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Related Experiment Videos

Human telomerase reverse transcriptase (hTERT) gene expression in thyroid neoplasms.

M Saji1, S Xydas, W H Westra

  • 1Department of Surgery, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287, USA. motosaji@welchlink.welch.jhu.edu

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
|July 2, 1999
PubMed
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Human telomerase reverse transcriptase (hTERT) gene expression is a promising marker for diagnosing indeterminate thyroid nodules. Measuring hTERT in fine-needle aspiration samples may improve accuracy in distinguishing benign from malignant thyroid lesions.

Area of Science:

  • Oncology
  • Molecular Biology
  • Endocrinology

Background:

  • Thyroid fine-needle aspirations (FNAs) often yield indeterminate results, necessitating further diagnostic markers.
  • Most indeterminate thyroid nodules are benign, highlighting the need for improved diagnostic accuracy.
  • Human telomerase reverse transcriptase (hTERT), the catalytic component of telomerase, is reactivated in immortalized cells.

Purpose of the Study:

  • To investigate the utility of hTERT gene expression as a marker for differentiating benign from malignant thyroid neoplasms.
  • To assess the correlation between hTERT gene expression and telomerase enzyme activity in thyroid nodules.
  • To determine if hTERT expression can be reliably measured in in vivo FNA samples.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect hTERT gene expression in thyroid neoplasms and nodules.

Related Experiment Videos

  • Telomerase enzyme activity was measured in all samples.
  • hTERT expression was analyzed in FNA samples.

    • Main Results:

    • hTERT gene expression was detected in 79% of malignant thyroid neoplasms versus 28% of benign thyroid nodules.
    • hTERT expression was absent in normal thyroid tissue.
    • hTERT gene expression correlated with telomerase activity in 95% of cases, with hTERT expression being a more sensitive marker in some papillary carcinomas.

    Conclusions:

    • hTERT gene expression is a potential biomarker for distinguishing malignant from benign thyroid lesions.
    • Measuring hTERT expression in FNA samples may enhance the diagnostic accuracy of suspicious thyroid nodules.
    • hTERT expression appears more accurate than telomerase activity for differentiating benign from malignant thyroid lesions.