Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Temporal modulation of GABA(A) receptor subunit gene expression in developing monkey cerebral cortex.

M M Huntsman1, A Muñoz, E G Jones

  • 1Department of Anatomy and Neurobiology, University of California, Irvine, 92697, USA.

Neuroscience
|July 3, 1999
PubMed
Summary

GABA(A) receptor subunit expression in macaque cortex increases during development, with specific patterns observed in fetal stages that differ from adults. These changes correlate with the formation of neural connections and developmental plasticity.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Natural history of human immunodeficiency virus type 1 viremia after seroconversion and proximal to AIDS in a large cohort of homosexual men. Multicenter AIDS Cohort Study.

The Journal of infectious diseases·2000
Same author

[Recurrent spontaneous carotid dissection].

Revista de neurologia·2000
Same author

Safety and efficacy of Omniscan (gadodiamide injection) at 0.1 mmol/kg for MRI in infants younger than 6 months of age: phase III open multicenter study.

Investigative radiology·2000
Same author

Percutaneous vertebroplasty: a special syringe for cement injection.

AJNR. American journal of neuroradiology·2000
Same author

OESCLIM: an advanced delivery system for HRT.

Maturitas·2000
Same author

Fenfluramine-induced increase in preproenkephalin mRNA levels in the striatum: interaction between the serotonergic, glutamatergic, and dopaminergic systems.

Synapse (New York, N.Y.)·2000

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • GABA(A) receptors are crucial inhibitory neurotransmitter receptors in the brain.
  • Understanding their developmental expression is key to comprehending brain maturation and plasticity.

Purpose of the Study:

  • To investigate the developmental expression patterns of ten GABA(A) receptor subunits in the macaque monkey cerebral cortex.
  • To compare fetal and postnatal expression with adult patterns.
  • To correlate expression changes with critical periods of neural connection formation.

Main Methods:

  • In situ hybridization histochemistry was employed to detect messenger RNA (mRNA) for GABA(A) receptor subunits.
  • Analysis focused on primary somatosensory and visual cortical areas.

Related Experiment Videos

  • Expression levels were examined across embryonic (E125-E150) and postnatal (P125) periods.

    • Main Results:

    • GABA(A) receptor subunit transcript levels were low in early fetal development, increasing significantly by E137, particularly in sensorimotor areas (3a, 3b) and specific cortical layers (III/IV, VI).
    • Postnatal development showed decreased transcript levels, yet alpha1, alpha5, beta2, and gamma2 subunits remained highly expressed, unlike in rodents.
    • Fetal visual cortex (area 17) exhibited unique lamina-specific expression patterns (e.g., alpha3, gamma1) not present in adults, with alpha5 peaking significantly at E150.

    Conclusions:

    • GABA(A) receptor expression is dynamically regulated during primate cortical development, coinciding with the establishment of thalamocortical and intracortical connections.
    • The observed temporal regulation suggests a role in developmental plasticity.
    • Unlike rodents, macaque cortex does not show a major subunit "switch" between fetal and adult stages, with certain subunits consistently dominant.