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IL-10 up-regulates CCR5 gene expression in human monocytes
M Houle1, M Thivierge, C Le Gouill
1Department of Pediatrics Faculty of Medicine, Université de Sherbrooke, Quebec, Canada.
Inflammation
|July 7, 1999
Summary
Interleukin-10 (IL-10) up-regulates the chemokine receptor CCR5 in human monocytes, impacting inflammation and HIV-1 infection susceptibility. This cytokine modulation of CCR5 expression is a key regulatory mechanism.
Area of Science:
- Immunology
- Molecular Biology
- Virology
Background:
- The chemokine receptor CCR5 is crucial for macrophage-tropic HIV-1 infection.
- CCR5 deficiency confers resistance to HIV-1.
- Cytokines regulate immune cell function, including CCR5 expression.
Purpose of the Study:
- To investigate the role of interleukin-10 (IL-10) in regulating CCR5 gene expression in human monocytes.
- To determine the functional consequences of IL-10-mediated CCR5 modulation.
Main Methods:
- Human monocytes were treated with varying concentrations of IL-10.
- CCR5 mRNA and protein expression were quantified using RT-PCR and antibody labeling.
- Monocyte function, including calcium mobilization and chemotaxis, was assessed in response to CCR5 ligands.
Main Results:
- IL-10 rapidly and dose-dependently up-regulated CCR5 mRNA and protein expression in monocytes.
- The IL-10 effect on CCR5 expression was mediated transcriptionally.
- IL-10 treatment enhanced monocyte intracellular calcium mobilization and chemotaxis towards MIP-1beta.
Conclusions:
- Interleukin-10 significantly modulates CCR5 expression and function in human monocytes.
- This IL-10-driven regulation of CCR5 may influence inflammatory responses.
- Modulation of CCR5 by IL-10 could impact susceptibility to HIV-1 infection.