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Updated: Jul 30, 2026

Automated Protocols for Macromolecular Crystallization at the MRC Laboratory of Molecular Biology
Published on: January 24, 2018
Macromolecular crystal annealing: evaluation of techniques and variables
J M Harp1, B L Hanson, D E Timm
1University of Tennessee/Oak Ridge, Graduate School of Biomedical Sciences, USA.
Abstract:
Additional examples of successful application of macromolecular crystal annealing are presented. A qualitative evaluation of variables related to the annealing process was conducted using a variety of macromolecular crystals to determine in which cases parameters may be varied and in which cases the original macromolecular crystal annealing protocol is preferred. A hypothesis is presented relating the solvent content of the crystal to the specific protocol necessary for the successful application of annealing.

