Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reconstructed human cornea produced in vitro by tissue engineering.

L Germain1, F A Auger, E Grandbois

  • 1Laboratoire d'Organogénèse Expérimentale (LOEX), Hôpital du Saint-Sacrement, Québec et Département de Chirurgie, Université Laval, Sainte-Foy, Canada. lucie.germain@chg.ulaval.ca

Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology
|July 8, 1999
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Dialogue between general practitioner and patient regarding tobacco and alcohol consumption, from the patient's standpoint].

Revue d'epidemiologie et de sante publique·2020
Same author

The use of tissue-engineered skin to demonstrate the negative effect of CXCL5 on epidermal ultraviolet radiation-induced cyclobutane pyrimidine dimer repair efficiency.

The British journal of dermatology·2020
Same author

Urogenital symptoms in mitochondrial disease: overlooked and undertreated.

European journal of neurology·2019
Same author

The Red Kangaroo pericardium as a material source for the manufacture of percutaneous heart valves.

Morphologie : bulletin de l'Association des anatomistes·2019
Same author

Autologous bilayered self-assembled skin substitutes (SASSs) as permanent grafts: a case series of 14 severely burned patients indicating clinical effectiveness.

European cells & materials·2018
Same author

[Smoking and vaping in France].

Revue des maladies respiratoires·2018

Researchers engineered a functional human cornea in vitro, successfully demonstrating key basement membrane proteins and integrins. This tissue-engineered cornea model is valuable for future research and drug testing.

Area of Science:

  • Biomedical Engineering
  • Ophthalmology
  • Tissue Engineering

Background:

  • The development of in vitro models for human cornea is crucial for research and drug development.
  • Understanding the expression of specific proteins in engineered tissues is essential for validating their functionality.

Purpose of the Study:

  • To create a tissue-engineered human cornea in vitro.
  • To characterize the expression of integrins and basement membrane proteins in the engineered cornea.

Main Methods:

  • Human corneal epithelial cells and fibroblasts were isolated and cultured.
  • Reconstructed corneas were generated by culturing epithelial cells on fibroblast-laden collagen gels.
  • Histological and immunohistological analyses were performed to assess tissue structure and protein expression.

Related Experiment Videos

Main Results:

  • Limbus-derived corneal epithelial cells showed superior growth characteristics in vitro compared to central cells.
  • The engineered cornea exhibited appropriate histology with 4-5 cell layers by day 3.
  • Basement membrane components and various integrin subunits were detected by day 3, primarily at the epithelium-stroma junction.

Conclusions:

  • The in vitro tissue-engineered human cornea demonstrates suitable histology and expresses key basement membrane proteins and integrins.
  • This engineered model serves as a valuable tool for physiological, toxicological, and pharmacological studies.
  • The model is also promising for gene expression studies in corneal research.