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HLA-B*4703: sequence confirmation, serology and distribution.

C Darke1, M G Guttridge, J Street

  • 1Regional Tissue Typing Laboratory, Welsh Blood Service, Cardiff, United Kingdom. cdarke@wbmdr.demon.co.uk

Tissue Antigens
|July 8, 1999
PubMed
Summary

The B*4703 allele, identified by unique HLA-B47 and Bw6 patterns, was confirmed in Black individuals. This allele is distinct from HLA-B47 and rare in Northern European populations.

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Area of Science:

  • Immunogenetics
  • Human Leukocyte Antigen (HLA) system

Background:

  • The Human Leukocyte Antigen (HLA) system plays a crucial role in immune response and transplantation.
  • Unusual serological patterns can indicate novel HLA alleles requiring detailed characterization.

Purpose of the Study:

  • To confirm the nucleotide sequence of the novel B*4703 allele.
  • To differentiate B*4703 from established HLA-B47 specificities.
  • To investigate the frequency and associations of B*4703 in different ethnic groups.

Main Methods:

  • Nucleotide sequencing of exons 2 and 3 of the B*4703 allele.
  • HLA-B47 cross-reactive sera titration studies and Bw6 antisera/monoclonal antibody testing.
  • Polymerase Chain Reaction Sequence Specific Priming (PCR-SSP) for HLA-A,B typing.

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Main Results:

  • The nucleotide sequence of B*4703 was confirmed in individuals of Black/Japanese and Caribbean Black descent.
  • B*4703 was distinguished from the established HLA-B47 specificity through serological analysis.
  • An association between B*4703 and Cw*0701/06 was suggested in Black subjects.
  • The phenotype frequency of B*4703 was found to be less than 0.0098% in a Northern European Caucasoid population.

Conclusions:

  • The B*4703 allele is a distinct HLA-B allele confirmed by its nucleotide sequence and serological characteristics.
  • B*4703 appears to be rare in Northern European populations but may have specific associations in Black populations.