Identification of phosphate-regulated genes by differential expression in the UV-irradiated host system
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1Division of Cancer Research, National Health Research Institutes, Taipei, Taiwan, ROC. sechuang@nhri.org.tw
Journal of Microbiological Methods
|July 8, 1999
Abstract:
The UV-irradiated host system has been used for identifying protein products of genes cloned in a phage vector. By starving the host cells for phosphate immediately before UV-irradiation, we demonstrate that phosphate-regulated genes can be easily identified. By employing this new technique, we also provide evidence showing that the gpsA gene might be a new member of the phosphate starvation-inducible (psi) genes of E. coli.
