Interference of linoleic acid fraction in some receptor binding assays
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Updated: Oct 20, 2026
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
Published on: November 17, 2011
K Ingkaninan1, J K von Frijtag Drabbe Künzel, A P IJzerman
1Division of Pharmacognosy and Division of Medicinal Chemistry, Leiden/Amsterdam Center for Drug Research, Gorlaeus Laboratories, Leiden University, P.O. Box 9502, 2300 RA, Leiden, The Netherlands. ingkanin@chem.leidenuniv.nl
Abstract:
An extract of a suspension culture of Tabernaemontana pandacaqui Poir. was fractionated by centrifugal partition chromatography. Aliquots were tested in an adenosine A1 receptor binding assay. This led to the isolation and identification of linoleic acid, which proved to be a noncompetitive inhibitor. This "false positive"effect also extended to some other binding assays.
