Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immune system development and function in prolactin receptor-deficient mice.

B Bouchard1, C J Ormandy, J P Di Santo

  • 1Institut National de la Santé et de la Recherche Médicale U-344, Endocrinologie Moléculaire, Faculté de Médecine Necker, Paris, France.

Journal of Immunology (Baltimore, Md. : 1950)
|July 8, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High Speed Spin Testing of Reinforced 2212 Coils for High Field NMR Magnets.

IEEE transactions on applied superconductivity : a publication of the IEEE Superconductivity Committee·2020
Same author

Bacterial virulence factor inhibits caspase-4/11 activation in intestinal epithelial cells.

Mucosal immunology·2016
Same author

Tuberculosis in Laos, who is at risk: the mahouts or their elephants?

Epidemiology and infection·2014
Same author

c-Myc and Her2 cooperate to drive a stem-like phenotype with poor prognosis in breast cancer.

Oncogene·2013
Same author

RNAi-mediated stathmin suppression reduces lung metastasis in an orthotopic neuroblastoma mouse model.

Oncogene·2013
Same author

Functional characterization of cancer-associated Gab1 mutations.

Oncogene·2012

Prolactin (PRL) signaling is not essential for immune system development or function. Studies in prolactin receptor-deficient mice show normal immune responses, indicating PRLR pathways are not required for immunomodulation in vivo.

Area of Science:

  • Immunology
  • Endocrinology
  • Molecular Biology

Background:

  • Prolactin (PRL) is a pituitary hormone crucial for reproduction and potentially involved in immunoregulation.
  • Previous studies suggested PRL might modulate the immune system, based on observations in hypophysectomized rats.

Purpose of the Study:

  • To investigate the role of prolactin receptor (PRLR) signaling in immune system development and function.
  • To determine if PRLR pathways are essential for in vivo immunomodulation.

Main Methods:

  • Characterization of immune system development and function in PRLR-deficient (PRLR-/-) mice.
  • Analysis of thymic and splenic cellularity, lymphocyte subset composition, and in vitro lymphocyte proliferation.
  • Assessment of NK-mediated cytotoxicity, Ig levels, specific antibody responses, tumor cell clearance, and Listeria monocytogenes infection control in vivo.

Related Experiment Videos

Main Results:

  • PRLR-/- mice showed no significant alterations in thymic or splenic cellularity or lymphocyte subset composition.
  • In vitro studies confirmed normal lymphocyte proliferation and NK-mediated cytotoxicity in PRLR-/- mice.
  • In vivo assessments revealed normal Ig levels, antibody responses, tumor cell elimination, and Listeria infection control in PRLR-/- mice.

Conclusions:

  • Immune system development and function are normal in the absence of PRLR-mediated signaling.
  • PRLR pathways are not essential for effective immunomodulation in vivo.