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Related Experiment Videos

Increased nitric oxide in infective gastroenteritis.

M Herulf1, B Svenungsson, A Lagergren

  • 1Department of Physiology and Pharmacology (Division of Pharmacology), Karolinska Institutet, 171 77 Stockholm, Sweden. max.herulf@fyfa.ki.se

The Journal of Infectious Diseases
|July 9, 1999
PubMed
Summary

Luminal nitric oxide (NO) is significantly elevated in patients with gastroenteritis, indicating increased gut inflammation. Measuring this NO via rectal sampling offers a new diagnostic tool for intestinal disease.

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Area of Science:

  • Gastroenterology
  • Biochemistry
  • Inflammation Research

Background:

  • Nitric oxide (NO) plays a role in inflammatory disorders, but its specific function in the gut remains unclear.
  • Investigating NO's role in infective gastroenteritis is crucial for understanding gut inflammation.

Purpose of the Study:

  • To determine if luminal nitric oxide (NO) levels are elevated in the intestine during infective gastroenteritis.
  • To assess the potential of measuring rectal NO as a diagnostic marker for gut inflammation.

Main Methods:

  • Rectal gas sampling using silicone balloon catheters in 17 gastroenteritis patients and 10 healthy volunteers.
  • Analysis of nitric oxide (NO) by chemiluminescence and plasma nitrate/nitrite levels by capillary electrophoresis.

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Main Results:

  • Luminal NO levels were dramatically higher in gastroenteritis patients (9441 ppb) compared to controls (74 ppb).
  • A significant increase in plasma nitrite, but not nitrate, was observed in patients.
  • Complete separation in NO levels between patients and controls suggests high diagnostic potential.

Conclusions:

  • Luminal nitric oxide is substantially increased in infective gastroenteritis.
  • Rectal NO measurement is a feasible and sensitive method for assessing local gut NO production.
  • This technique may serve as a valuable tool for evaluating intestinal inflammation in health and disease.