Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Eubacterial tmRNAs: everywhere except the alpha-proteobacteria?

B Felden1, R F Gesteland, J F Atkins

  • 1Department of Human Genetics, University of Utah, Salt Lake City, UT 84112-5330, USA. bfelden@genetics.utah.edu

Biochimica Et Biophysica Acta
|July 9, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An outbreak in intravenous drug users due to USA300 Latin-American variant community-acquired methicillin-resistant Staphylococcus aureus in France as early as 2007.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2017
Same author

Ribosomal frameshifting used in influenza A virus expression occurs within the sequence UCC_UUU_CGU and is in the +1 direction.

Open biology·2012
Same author

Theiler's murine encephalomyelitis virus contrasts with encephalomyocarditis and foot-and-mouth disease viruses in its functional utilization of the StopGo non-standard translation mechanism.

The Journal of general virology·2012
Same author

An overlapping protein-coding region in influenza A virus segment 3 modulates the host response.

Science (New York, N.Y.)·2012
Same author

Attenuation of an amino-terminal premature stop codon mutation in the ATRX gene by an alternative mode of translational initiation.

Journal of medical genetics·2004
Same author

Thermodynamic criteria for high hit rate antisense oligonucleotide design.

Nucleic acids research·2003
Same journal

Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Molecular Basis of Disease Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

General Subjects Cumulative Contents.

Biochimica et biophysica acta·2020
Same journal

Erratum to 'on the role of exchangeable hydrogen bonds for the kinetics of P680<sup>+·</sup> Q<sub>A</sub> <sup>-·</sup> formation and P680<sup>+·</sup> Pheo<sup>-·</sup> recombination in photosystem II' [Biochim. Biophys. Acta 1276 (1996) 35-44].

Biochimica et biophysica acta·2019
Same journal

Oligomeric state of the light-harvesting complexes B800-850 and B875 from purple bacterium Rubrivivax gelatinosus in detergent solution.

Biochimica et biophysica acta·2019
Same journal

Regulation of pigment content and enzyme activity in the cyanobacterium Nostoc sp. Mac grown in continuous light, a light-dark photoperiod, or darkness.

Biochimica et biophysica acta·2019
See all related articles

Eubacterial tmRNAs tag aberrant proteins for degradation, aiding ribosome recycling from faulty mRNA. This study determined 58 new tmRNA sequences, revealing evolutionary insights across bacterial phyla.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Eubacterial tmRNAs (transfer-messenger RNAs) are crucial for ribosome recycling when encountering terminatorless mRNAs.
  • They encode a peptide tag that targets aberrant proteins, produced from truncated mRNAs, for proteolysis.

Purpose of the Study:

  • To investigate the evolutionary diversity of tmRNA genes in Eubacteria.
  • To determine new tmDNA sequences and analyze their distribution across different bacterial phyla.

Main Methods:

  • Polymerase Chain Reaction (PCR) was employed to amplify Eubacterial tmRNA genes.
  • Sequencing of 58 newly amplified tmDNA fragments.

Main Results:

  • Fifty-eight novel tmDNA sequences were determined, expanding the known tmRNA repertoire.

Related Experiment Videos

  • tmDNA sequences were successfully amplified from members of nine additional bacterial phyla.
  • Notably, tmDNA sequences were absent in all tested species belonging to the alpha-Proteobacteria class.

    • Conclusions:

    • The findings highlight the widespread presence of tmRNA systems in Eubacteria, with significant evolutionary implications.
    • The absence of tmRNA in alpha-Proteobacteria suggests a potential loss or divergence of this system within this specific bacterial group.