Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tuning somatic hypermutation by transcription.

H Jacobs1, A Puglisi, K Rajewsky

  • 1Basel Institute for Immunology, Switzerland.

Current Topics in Microbiology and Immunology
|July 9, 1999
PubMed
Summary

Somatic hypermutation in immunoglobulin genes is dependent on transcription levels. Researchers found that increased gene transcription directly correlates with higher mutation frequencies in B cells, suggesting transcription can tune mutation load.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Rescaling invariance and anomalous energy transport in a small vertical column of grains.

Physical review. E·2023
Same author

Revealing the Nonequilibrium Nature of a Granular Intruder: The Crucial Role of Non-Gaussian Behavior.

Physical review letters·2023
Same author

Thermodynamic bounds for diffusion in nonequilibrium systems with multiple timescales.

Physical review. E·2023
Same author

Collective Drifts in Vibrated Granular Packings: The Interplay of Friction and Structure.

Physical review letters·2022
Same author

Vicsek model by time-interlaced compression: A dynamical computable information density.

Physical review. E·2021
Same author

Engineered swift equilibration of a Brownian gyrator.

Physical review. E·2020

Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Somatic hypermutation (SHM) is a critical process for adaptive immunity, generating antibody diversity.
  • The precise mechanisms regulating SHM, particularly the role of transcription, are not fully understood.

Purpose of the Study:

  • To investigate the dependence of somatic hypermutation on transcription.
  • To determine if transcription levels can modulate the mutation frequency of immunoglobulin genes.

Main Methods:

  • Utilized three mutant immunoglobulin heavy chain (IgH) insertion mouse models with a VHB1-8 passenger transgene.
  • Varied transcriptional control using truncated DQ52 (p delta), RNA polymerase II (pII), and RNA polymerase I (pI) promoters.
  • Quantified transgene mutation frequency in memory B cells and transgene-specific pre-mRNA levels in germinal center B cells.

Related Experiment Videos

Main Results:

  • Mutation frequency of the VHB1-8 transgene in memory B cells showed a strong correlation with pre-mRNA levels.
  • p delta, pI, and pII mice exhibited mutation frequencies of 7%, 60%, and 100%, respectively, mirroring pre-mRNA levels of 8%, 72%, and 100%.

Conclusions:

  • These findings indicate that the mutation load of rearranged immunoglobulin genes can be regulated by transcription.
  • Further investigation is ongoing to ascertain if SHM requires transcription per se or specific RNA polymerase II components.