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Saccharomyces cerevisiae MPS2 encodes a membrane protein localized at the spindle pole body and the nuclear envelope

M C Muñoz-Centeno1, S McBratney, A Monterrosa

  • 1Service de Biochimie et de Génétique Moléculaire, Commissariat à l'Energie Atomique/Saclay, F-91191 Gif-sur-Yvette, France.

Molecular Biology of the Cell
|July 9, 1999
PubMed

Insights

The MPS2 gene is crucial for spindle pole body (SPB) duplication in yeast. MPS2 protein is an integral membrane protein localized to the SPB, essential for its insertion into the nuclear envelope.

Area of Science:

  • Cell biology
  • Molecular genetics
  • Yeast genetics

Background:

  • The MPS2 gene is essential for spindle pole body (SPB) duplication in Saccharomyces cerevisiae.
  • SPB duplication is critical for proper mitotic growth and chromosome segregation.

Purpose of the Study:

  • To characterize the MPS2 gene and its encoded protein.
  • To determine the localization and function of the MPS2 protein in yeast cells.

Main Methods:

  • Gene cloning and protein characterization.
  • Biochemical fractionation and protein tagging (myc epitope).
  • Fluorescence microscopy (GFP fusion) and immunoelectron microscopy.

Main Results:

  • MPS2 encodes an essential 44-kDa integral membrane protein with coiled-coil regions.
  • MPS2 protein localizes to the SPB, with perinuclear distribution and distinct foci.
  • Null strains exhibit slow growth and abnormal ploidy, indicating MPS2's role in mitotic stability.

Conclusions:

  • Mps2p is an integral SPB component.
  • Mps2p is required for the insertion of the nascent SPB into the nuclear envelope.
  • This function is vital for maintaining genomic stability during cell division.

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