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Process intensification by direct product sequestration from batch fermentations: application of a fluidised bed,
1Biochemical Recovery Group, Centre for Bioprocess Engineering, School of Chemical Engineering, University of Birmingham, B15 2TT UK.
Biotechnology and Bioengineering
|July 9, 1999
Summary
Direct product sequestration (DPS) offers improved yield and quality for purifying yeast extracellular acid protease compared to conventional methods. This novel approach integrates purification directly into fermentation for enhanced bioprocessing efficiency.
Area of Science:
- Biotechnology
- Biochemical Engineering
- Microbial Fermentation
Background:
- Extracellular acid protease from Yarrowia lipolytica is a valuable bioproduct.
- Conventional purification involves multiple discrete steps: fermentation, broth clarification, and chromatography.
- Existing methods can be inefficient, impacting yield and product quality.
Purpose of the Study:
- To critically compare the efficacy of different primary purification strategies for Yarrowia lipolytica extracellular acid protease.
- To evaluate conventional purification versus fluidised bed adsorption techniques.
- To assess the advantages of direct product sequestration (DPS) integrated with fermentation.
Main Methods:
- Comparison of conventional purification (fermentation, clarification, anion exchange chromatography) with fluidised bed adsorption.
- Evaluation of fluidised bed adsorption directly interfaced with post-fermentation broth.
- Assessment of fluidised bed adsorption integrated directly with productive fermentations (DPS).
Main Results:
- Direct product sequestration (DPS) demonstrated superior performance in protease purification.
- DPS resulted in improved yield and enhanced molecular quality of the extracellular acid protease.
- Fluidised bed adsorption, particularly when integrated with fermentation, offers significant advantages over conventional methods.
Conclusions:
- Direct product sequestration (DPS) represents a more effective primary purification strategy for microbial extracellular bioproducts.
- The design and operation of DPS devices are crucial for optimizing yield and product quality.
- DPS technology has broad applicability for other extracellular bioproducts from microbial fermentations.