Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RNA virus fitness.

Domingo1, Menéndez-Arias, Holland

  • 1Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Universidad Autónoma de Madrid, Cantoblanco, 28049-Madrid, Spain.

Reviews in Medical Virology
|July 1, 1997
PubMed
Summary

RNA viruses exhibit high mutation rates, creating diverse viral quasispecies populations. These populations can rapidly adapt to antiviral drugs, posing challenges for effective treatment strategies.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discussion.

Ophthalmology·2001
Same author

Introduction

Microbes and infection·2000
Same author

Rare genomic changes as a tool for phylogenetics.

Trends in ecology & evolution·2000
Same author

Coding in the song of the wren: importance of rhythmicity, syntax and element structure.

Animal behaviour·2000
Same author

Incorporation of stable organic radicals of the tris(2,4, 6-trichlorophenyl)methyl radical series to pyrrole units as models for semiconducting polymers with high spin multiplicity. 1.

The Journal of organic chemistry·2000
Same author

Stereoselective 1,3-dipolar cycloadditions of a chiral nitrone derived from erythrulose. An experimental and DFT theoretical study

The Journal of organic chemistry·2000

Area of Science:

  • Virology
  • Molecular Biology
  • Genetics

Background:

  • RNA viruses are major pathogens with high mutation rates (10^-3 to 10^-5).
  • Their replication lacks efficient proofreading, leading to genetic heterogeneity.
  • Populations exist as dynamic mutant swarms known as viral quasispecies.

Purpose of the Study:

  • To explore the genetic organization and adaptive capabilities of RNA viruses.
  • To understand the implications of viral quasispecies for vaccine and antiviral resistance.
  • To investigate the pre-existence of drug-resistant mutations in viral populations.

Main Methods:

  • Analysis of mutation rates in RNA virus replication.
  • Characterization of viral quasispecies dynamics.
  • Examination of fitness changes under different population passages.
  • Investigation of drug resistance in HIV-1 populations.

Main Results:

  • RNA viruses form heterogeneous quasispecies with statistically defined genomes.
  • Fitness can be lost through bottleneck events but gained via large population passages.
  • HIV-1 quasispecies exhibit pre-existing mutations conferring resistance to antiretroviral inhibitors.
  • Viral quasispecies can overcome selective pressures from vaccines and antiviral agents.

Conclusions:

  • The inherent genetic instability of RNA viruses drives rapid adaptation.
  • Viral quasispecies dynamics are crucial for understanding pathogen evolution and treatment outcomes.
  • Anticipating sustained inhibition of RNA viruses like HIV-1 without resistance emergence remains a significant challenge.

Related Experiment Videos