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Updated: Oct 19, 2026

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
Published on: August 12, 2019
Cloning and sequence analysis of the hemB gene of Rhodothermus marinus
K Gudmundsdóttir1, E Sigurdsson, S I Thorbjarnardóttir
1Laboratory of Molecular Genetics, Institute of Biology, University of Iceland, IS-108 Reykjavík, Iceland.
Abstract:
A Rhodothermus marinus gene, hemB, coding for 5-aminolevulinic acid (ALA) dehydratase (ALAD) has been cloned and sequenced. The reading frame of the hemB gene is 1020 base pairs encoding a protein of 340 amino acids with a calculated molecular mass of 37.4 kDa. The amino acid sequence shows homology with eubacterial and eukaryotic ALA dehydratases. A putative metal-binding site of the protein shows strongest homology with corresponding sites from plant ALA dehydratases that require Mg2+ for activity. It differs with respect to only one amino acid out of 20 from a corresponding site in pea ALAD.

