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Related Experiment Videos

Does adrenaline modulate the Na+-Ca2+ exchanger in isolated toad pacemaker cells?

Y K Ju1, D G Allen

  • 1Department of Physiology, University of Sydney (F13), Sydney, NSW 2006, Australia.

Pflugers Archiv : European Journal of Physiology
|July 10, 1999
PubMed
Summary

Beta-adrenergic stimulation increases intracellular calcium and firing rate in toad heart pacemaker cells. The sodium-calcium exchanger current increase is due to elevated calcium, not altered exchanger properties.

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Area of Science:

  • Cardiology
  • Cell Physiology
  • Pharmacology

Background:

  • Beta-adrenergic stimulation affects cardiac pacemaker cells, influencing intracellular calcium and heart rate.
  • The sodium-calcium exchanger (Na+-Ca2+ exchanger) plays a role in regulating intracellular calcium and cardiac function.

Purpose of the Study:

  • To investigate the effect of beta-adrenergic stimulation on the Na+-Ca2+ exchanger current (INa-Ca) in toad heart pacemaker cells.
  • To determine whether the observed increase in INa-Ca is caused by elevated intracellular calcium ([Ca2+]i) or altered Na+-Ca2+ exchanger properties.

Main Methods:

  • Single, isolated, voltage-clamped pacemaker cells from the cane toad (Bufo marinus) were used.
  • Intracellular calcium ([Ca2+]i) and membrane currents were measured.

Related Experiment Videos

  • Rapid caffeine application was used to assess the relationship between INa-Ca and [Ca2+]i.

    • Main Results:

    • Beta-adrenergic stimulation increased both intracellular calcium ([Ca2+]i) and the Na+-Ca2+ exchanger current (INa-Ca).
    • The relationship between INa-Ca and [Ca2+]i was linear during caffeine-induced calcium decline and unaffected by beta stimulation.
    • This indicates that the increase in INa-Ca is a consequence of elevated [Ca2+]i.

    Conclusions:

    • Beta-adrenergic stimulation enhances INa-Ca in pacemaker cells, contributing to increased firing rate.
    • The increase in INa-Ca is primarily driven by elevated intracellular calcium levels.
    • The intrinsic properties of the Na+-Ca2+ exchanger do not appear to change significantly under beta-adrenergic stimulation in this context.