Related Experiment Video

Updated: Oct 19, 2026

Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy

Monitoring Plasmid Replication in Live Mammalian Cells over Multiple Generations by Fluorescence Microscopy

Published on: December 13, 2012

Direct visualisation of plasmid DNA in individual chromatography adsorbent particles by confocal scanning laser

A Ljunglöf¹, P Bergvall, R Bhikhabhai

¹Amersham Pharmacia Biotech, Uppsala, Sweden. anders.ljunglof@eu.apbiotech.com

Journal of Chromatography. A

|July 10, 1999

Abstract:

Confocal microscopy was used for the measurement of plasmid DNA adsorbed to individual adsorbent particles intended for anion-exchange and triple helix affinity chromatography. Plasmid DNA was visualized with the fluorescent dye YOYO-1, that forms a highly fluorescent complex with double stranded DNA. Confocal images were translated into fluorescence intensity profiles and the distribution of plasmid DNA in the particles was measured. The results that adsorption of plasmid DNA mainly takes place in an outer layer of the particles. The described procedure can also be advantageously used to demonstrate triple helix formation between plasmid DNA and immobilized oligonucleotides.

More Related Videos

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'

Published on: May 26, 2013

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking

Published on: March 10, 2014

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Articles linked to this work by shared authors, journal, and citation graph.

Uptake and depuration of gold nanoparticles in Daphnia magna.

Ecotoxicology (London, England)·2014

An exclusion mechanism in ion exchange chromatography.

Biotechnology and bioengineering·2006

Use of dimensionless residence time to study variations in breakthrough behaviour in expanded beds formed from varied particle size distributions.

Biotechnology and bioengineering·2004

Hydrophobic interaction ligand selection and scale-up of an expanded bed separation of an intracellular enzyme from Saccharomyces cerevisiae.

Journal of chromatography. A·2002

Measurement of ligand distribution in individual adsorbent particles using confocal scanning laser microscopy and confocal micro-Raman spectroscopy.

Journal of chromatography. A·2000

The use of rapid on-line monitoring of products and contaminants from within an expanded bed to control separations exhibiting fast breakthrough characteristics and to maximize productivity.

Biotechnology and bioengineering·2000

See all related articles