Related Experiment Video
Updated: Oct 19, 2026

In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster
Published on: June 13, 2025
The integration machinery of ZAM, a retroelement from Drosophila melanogaster, acts as a sequence-specific
P Leblanc1, B Dastugue, C Vaury
1Unité INSERM U384, 63001 Clermont-Ferrand, France.
Abstract:
Retroviruses and retrotransposons insert into the host genome with no obvious sequence specificity. We examined the target sites of the retroelement ZAM by sequencing each host-ZAM junction in the genome of Drosophila melanogaster. Our overall data provide compelling evidence that ZAM integration machinery recognizes and leads to ZAM insertion into the sequence 5'-GCGCGCg-3'. This unique property of ZAM will facilitate the development of new tools to study the integration process of retroelements.
Related Concept Videos
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Non-LTR Retrotransposons
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Homologous Recombination
LTR Retrotransposons
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...

