Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Thyroid-specific gene expression in the multi-step process of thyroid carcinogenesis.

P Ros1, D L Rossi, A Acebrón

  • 1Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.

Biochimie
|July 13, 1999
PubMed
Summary

Thyroid transcription factors TTF-1 and Pax-8 are crucial for thyroid cell identity. Their expression and that of target genes decrease with thyroid cancer progression, offering potential prognostic markers.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Differentiation of Hemorrhage from Iodine Using Spectral Detector CT: A Phantom Study.

AJNR. American journal of neuroradiology·2018
Same author

Resveratrol alleviates ethanol-induced hormonal and metabolic disturbances in the rat.

Physiological research·2016
Same author

Intraspecific divergence and convergence of floral tube length in specialized pollination interactions.

Proceedings. Biological sciences·2014
Same author

[Severe type A insulin resistance syndrome due to a mutation in the insulin receptor gene].

Anales de pediatria (Barcelona, Spain : 2003)·2014
Same author

Clinical evaluation of rapid point-of-care testing for detection of novel influenza A (H1N1) virus in a population-based study in Spain.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases·2011
Same author

Glibenclamide treatment in relapsed transient neonatal diabetes as a result of a KCNJ11 activating mutation (N48D).

Diabetic medicine : a journal of the British Diabetic Association·2009

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • Thyroid-specific transcription factors, including TTF-1 and Pax-8, regulate key genes like thyroglobulin (Tg), thyroperoxidase (TPO), and the thyrotropin receptor (TSH-R).
  • These factors are essential for maintaining the differentiated cellular phenotype in the thyroid gland.

Purpose of the Study:

  • To investigate the expression patterns of TTF-1, Pax-8, and their target genes in various thyroid neoplasms.
  • To correlate the expression levels of these markers with the degree of tumor differentiation and potentially identify prognostic indicators for thyroid cancer.

Main Methods:

  • Analysis of TTF-1 and Pax-8 expression in surgical and fine needle aspiration (FNA) biopsy samples of thyroid tumors, including adenomas, papillary carcinomas, follicular carcinomas, anaplastic carcinomas, and medullary carcinoma (MTC).

Related Experiment Videos

  • Assessment of target gene expression (Tg, TPO, TSH-R) in the same samples.
  • Evaluation of promoter activities of Tg, TPO, and TSH-R in thyroid carcinoma cells.
  • Cotransfection experiments using expression vectors for TTF-1 and Pax-8 to study their effect on gene transcription.

    • Main Results:

    • TTF-1 and Pax-8 expression was observed in well-differentiated adenomas, decreasing in papillary and follicular carcinomas, and absent in anaplastic carcinomas.
    • Expression levels of Tg, TPO, and TSH-R paralleled those of TTF-1 and Pax-8.
    • TSH-R and TTF-1 gene expression were detected in medullary carcinoma (MTC) samples.
    • Thyroid-specific gene expression and transcription factor presence were lost in follicular, papillary, and anaplastic human carcinoma cells, with absent promoter activities.
    • Cotransfection with TTF-1 and Pax-8 vectors stimulated transcription of these genes to varying degrees.

    Conclusions:

    • The expression of TTF-1 and Pax-8, along with their target genes, is significantly reduced or lost in dedifferentiated thyroid cancers.
    • The observed correlation between marker expression and tumor differentiation suggests their potential clinical utility in evaluating and predicting the prognosis of thyroid cancer.
    • The presence of TSH-R and TTF-1 in MTC warrants further investigation into their specific roles in this tumor type.