Vaccine potential of 56-66 kDa protease secreted by Entamoeba histolytica

Related Experiment Video

Updated: Oct 19, 2026

Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection

Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection

Published on: June 12, 2014

S Gupta¹, S Naik, S R Naik

¹Department of Gastroenterology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow.

The Indian Journal of Medical Research

|July 14, 1999

Abstract:

Excretory/secretory (ES) antigens and sub-cellular fractions of E. histolytica (HM1:IMSS strain) were tested for the presence of common proteases using substrate gel electrophoresis. We obtained two E. histolytica proteases (56-66 kDa and 29 kDa) from ES material, soluble components and plasma membrane. Protease 56-66 kDa from ES antigen was selected for immunizing hamsters because it gave a consistent broad band. We observed 62.5 per cent protection in immunized animals, compared to 0 per cent in unimmunized controls. Although all vaccinated golden hamsters showed high antibody response, there was no correlation between antibody titres and protection. 56-66 kDa ES protease could thus prevent disease and could be a candidate molecular vaccine against amoebiasis.

More Related Videos

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

Published on: May 6, 2015

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

Related Concept Videos

Amebiasis

Amebiasis

Entamoeba histolytica, a protozoan parasite, is responsible for intestinal and extraintestinal amebiasis. Though a significant proportion of infections remain asymptomatic, approximately 50 million individuals annually are estimated to present with clinical disease, resulting in up to 100,000 deaths globally. The disease burden is disproportionately high in regions with lower socioeconomic status, such as parts of India, Africa, Mexico, and Latin America.Etiology and TransmissionThe infective...

Poliomyelitis

Poliomyelitis

Poliomyelitis is caused by poliovirus, a small, non-enveloped, positive-sense RNA virus of the Picornaviridae family and Enterovirus genus. Transmission occurs primarily via the fecal-oral route, often through ingestion of contaminated water or food. The virus initially replicates in the oropharynx and intestinal mucosa, particularly in lymphoid tissues such as the tonsils, Peyer’s patches, and regional lymph nodes. Primary viremia follows, allowing dissemination throughout the body.In most...

Vaccinations

Vaccinations

Articles linked to this work by shared authors, journal, and citation graph.

Signal transduction within the nucleus by mitogen-activated protein kinase.

The Journal of biological chemistry·1992

Occurrence and mechanisms of glycopeptide resistance in gram-positive cocci.

Infectious agents and disease·1992

Preferential expression and activity of multidrug resistance gene 1 product (P-glycoprotein), a functionally active efflux pump, in human CD8+ T cells: a role in cytotoxic effector function.

Journal of clinical immunology·1992

Changing profile of enteric fever--in summer-91.

The Journal of the Association of Physicians of India·1992

Characterization of Staphylococcus aureus isolates with decreased susceptibility to vancomycin and teicoplanin: isolation and purification of a constitutively produced protein associated with decreased susceptibility.

The Journal of infectious diseases·1992

Interrelationship between ovine follicular fluid inhibin and serum albumin.

Indian journal of experimental biology·1992

See all related articles