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Related Experiment Videos

Diagnostic electron microscopy is still a timely and rewarding method.

S S Biel1, H R Gelderblom

  • 1Robert Koch-Institut, Konsiliarlaboratorium für elektronenmikroskopische Erregerdiagnostik, Berlin, Germany.

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
|July 16, 1999
PubMed
Summary

Electron microscopy (EM) in virology offers rapid, open-view diagnostics. External Quality Assessment on EM virus diagnosis (EQA-EMV) programs are crucial for maintaining high standards and ensuring the continued performance of this valuable technique.

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Area of Science:

  • Virology
  • Electron Microscopy
  • Diagnostic Techniques

Background:

  • Electron microscopy (EM) has been integral to virology since the 1930s, evolving from early smallpox diagnosis to routine use by the 1960s.
  • Diagnostic EM was widely applied to various biological specimens, including cell cultures and clinical samples.
  • The advent of molecular and genetic techniques led to a decline in diagnostic EM's utilization.

Purpose of the Study:

  • To provide a perspective on the future applications and potential uses of diagnostic EM by examining its historical and current roles.
  • To describe the External Quality Assessment on EM virus diagnosis (EQA-EMV) system, established in 1994, and highlight its achievements.

Main Methods:

  • The External Quality Assessment on EM virus diagnosis (EQA-EMV) program was implemented to evaluate, confirm, and enhance the quality of diagnostic EM.

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  • The program distributes two types of specimens: prepared grids for assessing diagnostic skills and stabilized virus suspensions for evaluating preparation efficiency.

    • Main Results:

    • Diagnostic EM is characterized by its speed and comprehensive, undirected visualization capabilities.
    • The study emphasizes the advantages of diagnostic EM and discusses its indications for future adaptation.
    • Six years of EQA-EMV results are presented, underscoring the need for quality control measures to maintain high diagnostic standards.

    Conclusions:

    • Despite its advantages, the use of diagnostic EM in virology has decreased since the 1990s due to the rise of modern diagnostic methods.
    • External Quality Assessment (EQA) programs are essential for ensuring the continued performance and reliability of diagnostic EM.
    • The increasing number of participants and countries involved in EQA-EMV signifies a strong interest and need for such quality assurance initiatives.