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A mutational study of a Diels-Alderase catalytic antibody
1Howard Hughes Medical Institute and the Department of Chemistry, University of California, Berkeley 94720-1460, USA.
Bioorganic & Medicinal Chemistry Letters
|July 16, 1999
Abstract:
Site-directed mutagenesis was used to probe the mechanism of antibody 39-A11, which catalyzes the Diels-Alder reaction of substrate 1 and 2 to give cycloadduct 3. Mutations that lead to improved packing interactions with the diene afford an order of magnitude increase in catalytic activity.