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Interaction between explosive and analyte layers in explosive matrix-assisted plasma desorption mass spectrometry
1Ion Physics Division, Ångström Laboratory, Uppsala University, Box 534, SE-751 21 Uppsala, Sweden.
Rapid Communications in Mass Spectrometry : RCM
|July 17, 1999
Summary
This study on explosive materials for protein analysis in plasma desorption mass spectrometry revealed that increased charge state is a key matrix effect. A chemical energy release mechanism, not a simple interface, is proposed for explosive matrix assistance.
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Biochemistry
Background:
- Plasma desorption mass spectrometry (PDMS) is a technique used for analyzing biomolecules.
- Understanding matrix effects is crucial for optimizing protein analysis in PDMS.
Purpose of the Study:
- To investigate the matrix properties of explosive materials for protein analytes in PDMS.
- To elucidate the mechanism of explosive matrix assistance in protein analysis.
Main Methods:
- Utilized an HMX/insulin two-layer system as a model.
- Studied molecular ion yield and average charge state as a function of analyte thickness.
- Compared results with matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI).
Main Results:
- An increased charge state of multiply protonated species was identified as the primary matrix effect.
- Observed narrower charge state distributions than Poisson distributions, indicating limited protonation.
- Maximum ion and charge yields occurred at thicknesses other than a monolayer, suggesting a non-interface mechanism.
Conclusions:
- The findings suggest a mechanism involving chemical energy release from the explosive post-ion impact, rather than a simple matrix/analyte interface.
- Enhanced protonation and electron scavenging by explosive decay products may contribute to protein ion survival.