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Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
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Streamlined Purification of Plasmid DNA From Prokaryotic Cultures

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Rapid, economical filter alternative to chromosomal DNA centrifugation in the alkaline lysis plasmid protocol

M T McNally1

  • 1Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee 53226. mtm@mcw.edu

Biotechniques
|July 17, 1999
PubMed
Abstract

No abstract available in PubMed .

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DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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