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Intestinal plasma membrane calcium pump protein and its induction by 1,25(OH)(2)D(3) decrease with age.

H J Armbrecht¹, M A Boltz, V B Kumar

¹Geriatric Research, Education, and Clinical Center, St. Louis Veterans Affairs Medical Center, St. Louis 63125, USA.

The American Journal of Physiology

|July 17, 1999

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Aging significantly reduces intestinal calcium absorption by decreasing plasma membrane calcium pump protein levels. This decline impairs vitamin D

Area of Science:

Molecular Biology
Gastroenterology
Nutritional Science

Background:

The plasma membrane calcium pump in intestinal absorptive cells is crucial for vitamin D-dependent calcium transport.
Intestinal calcium absorption naturally declines with aging.

Purpose of the Study:

To investigate whether decreased expression of the plasma membrane calcium pump protein correlates with age-related decline in intestinal calcium transport.
To assess the impact of aging on the induction of the calcium pump by 1,25-dihydroxyvitamin D3.

Main Methods:

Western blotting was used to measure plasma membrane calcium pump protein levels in Fischer 344 rats at 2, 12, and 24 months of age.
Rats were rendered deficient in 1,25-dihydroxyvitamin D3 using a high-strontium diet, followed by administration of 1,25-dihydroxyvitamin D3 or vehicle.

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Main Results:

Calcium pump protein levels dropped by 90% in the duodenum and 65% in the ileum between 2 and 12 months of age, coinciding with a marked decrease in active calcium transport.
In 12-month-old rats, 1,25-dihydroxyvitamin D3 induced significantly less duodenal calcium pump protein (39%) and active calcium transport (33%) compared to 2-month-old rats.

Conclusions:

Decreased expression of plasma membrane calcium pump protein and calbindin protein parallels the age-related decline in intestinal calcium transport.
The responsiveness of intestinal calcium transport and calcium pump expression to 1,25-dihydroxyvitamin D3 is diminished with age.