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Related Experiment Videos

Polycystin-2 expression is developmentally regulated.

G S Markowitz1, Y Cai, L Li

  • 1Department of Pathology, Renal Pathology Laboratory, Columbia Presbyterian Medical Center, New York 10032, USA.

The American Journal of Physiology
|July 17, 1999
PubMed
Summary
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PKD2, a gene mutated in autosomal dominant polycystic kidney disease (ADPKD), shows widespread early expression in mouse embryos, suggesting a key developmental role. Its persistent expression in adult kidneys indicates a function in maintaining mature kidney tubules.

Area of Science:

  • Developmental Biology
  • Genetics
  • Nephrology

Background:

  • Mutations in PKD2 are implicated in 15% of autosomal dominant polycystic kidney disease (ADPKD) cases.
  • The function of the protein encoded by PKD2 remains largely unknown.
  • Understanding PKD2 expression patterns is crucial for elucidating its role in kidney development and disease.

Purpose of the Study:

  • To investigate the spatio-temporal expression pattern of Pkd2 during mouse embryonic and postnatal development.
  • To identify potential roles of Pkd2 in kidney development and other organ systems.

Main Methods:

  • Utilized polyclonal antisera against PKD2 for immunohistochemical staining.
  • Examined Pkd2 expression in staged mouse embryos (from E6) and postnatal tissues.

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  • Analyzed expression patterns in various tissues, including kidneys, adrenal cortex, endocrine organs, and muscle.

    • Main Results:

    • PKD2 expression was detected as early as embryonic day 6 (E6) in ectoderm and endoderm.
    • Expression in the kidney was initially low in the ureteric bud (E12.5) and established an adult pattern by E15.5 (proximal tubules: low; distal tubules: high, basolateral).
    • Significant expression was observed in fetal adrenal cortex, red blood cell precursors, endocrine organs, muscle, and mesenchymal tissues, with restricted expression postnatally.

    Conclusions:

    • The diffuse and early expression of Pkd2 suggests a fundamental role in embryonic development.
    • Persistent strong expression in adult kidney tubules indicates an organ-specific function in maintaining mature kidney structure.
    • PKD2's broad expression profile points to diverse roles beyond kidney development, potentially impacting multiple organ systems.