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Determination of leukotriene E4 in human urine using liquid chromatography-tandem mass spectrometry
M Mizugaki1, T Hishinuma, N Suzuki
1Department of Pharmaceutical Science, Tohoku University Hospital, Sendai, Japan. mizugaki@mail.cc.tohoku.ac.jp
Abstract:
A liquid chromatographic-tandem mass spectrometric (LC-MS-MS) method was developed for the quantitation of urinary leukotriene E4 (LTE4). LTE4 and its internal standard were extracted by solid-phase extraction and analysed using LC-MS-MS in the selected reaction monitoring (SRM) mode. A good linear response over the range of 10 pg to 10 ng was demonstrated. The accuracy of added LTE4 ranged from 97.0% to 108.0% with a mean and SD of 100.6+/-2.4%. We detected LTE4 (63.1+/-18.7 pg/mg creatinine, n=10) in healthy human urine. This method can be used to determine LTE4 in biological samples.