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High-performance liquid chromatographic purification and capillary electrophoresis quantification of the chemokine
C Arcelloni1, A Aiuti, A Cipponi
1Dept. of Laboratory Medicine, IRCCS, H S. Raffaele, Milan, Italy. arcelloni.cinzia@hsr.it
Abstract:
Chemokines are members of the chemotactic cytokines family implicated in various immunoregulatory functions. The CXC-chemokine stromal cell derived factor-1 (SDF-1alpha) was purified from the culture medium of murine bone marrow stromal cell line (MS-5) by affinity and reversed-phase liquid chromatography. Yield and purity were assessed by capillary electrophoresis (CE) with reference to the human SDF-1alpha from recombinant DNA technology. CE technique was useful to evaluate the purity of human SDF-1alpha from chemical synthesis and to resolve murine and human SDF-1alpha, differing by only one amino acid. Chemotactic activity of the murine SDF-1alpha was tested on the basis of CE quantification.