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Oxidation of phenols by horseradish peroxidase and lactoperoxidase compound II--kinetic considerations
M S Zahida1, W Deva, G M Peerzada
1Department of Chemical Sciences, Tata Institute of Fundamental Research, Navy Nagar, Bombay.
Indian Journal of Biochemistry & Biophysics
|July 21, 1999
Abstract:
Oxidation of para substituted phenols by horseradish peroxidase compound II (HRP-II) and lactoperoxidase compound II (LPO-II) were studied using stopped flow technique. Apparent second order rate constants (kapp) of the reactions were determined. The kinetics of oxidation of phenols by HRP-II and LPO-II have been compared with the oxidation potentials of the substrates. Reorganization energies of electron-transfer of phenols to the enzymes were estimated from the variation of second order rate constants with the thermodynamic driving force.