Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Engineered alignment in media equivalents: magnetic prealignment and mandrel compaction.

V H Barocas1, T S Girton, R T Tranquillo

  • 1Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455-0132, USA.

Journal of Biomechanical Engineering
|July 21, 1999
PubMed
Summary

We used anisotropic biphasic theory to predict and measure smooth muscle cell (SMC) orientation in tissue engineering. Mandrel compaction enhanced SMC alignment and tissue stiffness, crucial for vascular tissue development.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Melanoma/CSPG4-Enhanced Collagen-Mediated Contact Guidance Requires Mutant Active BRAF and the CSPG4 Core Protein Cytoplasmic Domain.

Cellular and molecular bioengineering·2026
Same author

The Ring-Pull Assay for Mechanical Properties of Fibrous Soft Tissues - An Analysis of the Uniaxial Approximation and a Correction for Nonlinear Thick-Walled Tissues.

Experimental mechanics·2021
Same author

Crack Propagation Versus Fiber Alignment in Collagen Gels: Experiments and Multiscale Simulation.

Journal of biomechanical engineering·2015
Same author

Cross-Linked Fiber Network Embedded in Elastic Matrix.

Soft matter·2013
Same author

Multiscale mechanical simulations of cell compacted collagen gels.

Journal of biomechanical engineering·2013
Same author

Simulated remodeling of loaded collagen networks via strain-dependent enzymatic degradation and constant-rate fiber growth.

Mechanics of materials : an international journal·2011

Area of Science:

  • Biomedical Engineering
  • Tissue Engineering
  • Biomechanics

Background:

  • Smooth muscle cells (SMCs) play a critical role in vascular tissue mechanics.
  • Understanding SMC orientation is key for developing functional tissue equivalents.
  • Fabrication methods significantly influence cell behavior and tissue properties.

Purpose of the Study:

  • To predict and measure the evolution of SMC orientation in media-equivalents (MEs) under different fabrication conditions.
  • To investigate the effects of free vs. mandrel compaction and magnetic prealignment on SMC orientation.
  • To validate the anisotropic biphasic theory (ABT) for tissue-equivalent mechanics.

Main Methods:

  • Fabrication of MEs under four conditions: F-, M-, F+, M+ (Free/Mandrel compaction with/without magnetic prealignment).

Related Experiment Videos

  • Utilized anisotropic biphasic theory (ABT) for mechanical predictions.
  • Measured SMC orientation evolution within the MEs.

    • Main Results:

    • ABT successfully predicted trends in SMC orientation for all fabrication conditions.
    • Free compaction (F-) maintained initial isotropic state; F+ lost circumferential alignment.
    • Mandrel compaction (M-) developed alignment; M+ enhanced initial alignment.
    • Mandrel presence led to increased circumferential stiffness due to cell alignment and stress-induced matrix deposition.

    Conclusions:

    • Fabrication conditions, particularly mandrel compaction, significantly influence SMC orientation.
    • Mandrel compaction promotes circumferential SMC and collagen alignment, enhancing mechanical stiffness.
    • The study validates ABT in predicting cell behavior and tissue development in engineered tissues.