Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement receptor 1 red cell expression is not controlled by the In(Lu) gene.

J M Moulds1, C Shah

  • 1Division of Rheumatology and Clinical Immunogenetics, University of Texas-Houston Medical School, 77030, USA. moulds@heart.med.uth.tmc.edu

Transfusion
|July 21, 1999
PubMed
Summary

The In(Lu) gene suppresses Lutheran blood group antigens but not the Knops blood group system antigens or complement receptor 1 (CR1) expression on red blood cells. This indicates distinct genetic regulation for these antigen systems.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mammary myofibroblastoma of the male breast: a case report and literature review.

Annals of the Royal College of Surgeons of England·2024
Same author

Intensive Blood Pressure Management Preserves Functional Connectivity in Patients with Hypertension from the Systolic Blood Pressure Intervention Randomized Trial.

AJNR. American journal of neuroradiology·2023
Same author

Comparison between Simple and Classical Techniques to Create Closed Pneumoperitoneum.

Kathmandu University medical journal (KUMJ)·2022
Same author

A new benchmark of soft X-ray transition energies of <math></math> , <math></math> , and <math></math> : paving a pathway towards ppm accuracy.

The European physical journal. D, Atomic, molecular, and optical physics·2022
Same author

Association of clinical factors and recent anticancer therapy with COVID-19 severity among patients with cancer: a report from the COVID-19 and Cancer Consortium.

Annals of oncology : official journal of the European Society for Medical Oncology·2021
Same author

Absolute throughput calibration of multiple spherical crystals for the Orion High-REsolution X-ray spectrometer (OHREX).

The Review of scientific instruments·2021

Area of Science:

  • Hematology
  • Immunology
  • Genetics

Background:

  • The In(Lu) gene is known to suppress various blood group antigens, including those in the Knops system.
  • Complement receptor 1 (CR1) carries Knops system antigens and exhibits red blood cell (RBC) expression polymorphism.
  • Previous studies suggested a role for In(Lu) in Knops antigen expression, necessitating further investigation.

Purpose of the Study:

  • To re-evaluate the role of the In(Lu) gene in the expression of Knops blood group system antigens.
  • To determine if In(Lu) affects complement receptor 1 (CR1) expression on red blood cells.

Main Methods:

  • Phenotyping of nine Lu(a-b-) donors for Lutheran and Knops blood group antigens.
  • Immunoblotting to analyze CR1 and Lutheran glycoproteins.

Related Experiment Videos

  • Quantification of RBC CR1 expression using ELISA and genetic determination of CR1 alleles via Southern blot.

    • Main Results:

    • Lu(a-b-) donors showed suppressed Lutheran antigens but normal Knops antigen expression.
    • Two donors exhibited weak Lutheran glycoprotein bands; others had no detectable Lutheran glycoprotein.
    • All donors displayed normal CR1 expression and CR1 allele inheritance patterns.

    Conclusions:

    • The genes responsible for suppressing Lutheran antigens do not impact CR1 glycoprotein or its associated Knops blood group antigens.
    • This study clarifies the distinct genetic control over Lutheran and Knops blood group antigen expression.