Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Modulating dipoles for structure-function correlations in the gramicidin A channel.

M Cotten1, C Tian, D D Busath

  • 1Center for Interdisciplinary Magnetic Resonance at the National High Magnetic Field Laboratory, Department of Chemistry, Florida State University, Tallahassee 32310, USA.

Biochemistry
|July 22, 1999
PubMed
Summary

Fluorination of tryptophan indoles in gramicidin channels alters dipole orientation and magnitude, influencing ion conductance. These findings provide insights into electrostatic interactions governing ion transport through membrane proteins.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Consistency evaluation of the domestic Lifotronic platform and foreign luminescence platforms in detecting core cerebrospinal fluid biomarkers in Chinese Alzheimer disease patients].

Zhonghua yi xue za zhi·2026
Same author

Evidence for the Collective Nature of Radial Flow in Pb+Pb Collisions with the ATLAS Detector.

Physical review letters·2026
Same author

Evidence for the Dimuon Decay of the Higgs Boson in pp Collisions with the ATLAS Detector.

Physical review letters·2025
Same author

Evidence for Longitudinally Polarized W Bosons in the Electroweak Production of Same-Sign W Boson Pairs in Association with Two Jets in pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

Physical review letters·2025
Same author

Hyperoxemia and its impact on in-hospital mortality in intracerebral hemorrhage patients: A retrospective cohort study.

Revue neurologique·2025
Same author

<i>Steinernema tarimense</i> n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Tarim Basin, Xinjiang, China.

Journal of helminthology·2025

Area of Science:

  • Biophysics
  • Structural Biology
  • Membrane Protein Research

Background:

  • Tryptophan indole dipoles significantly affect ion conductance in gramicidin channels.
  • Understanding these dipole effects is crucial for elucidating ion transport mechanisms.

Purpose of the Study:

  • To investigate how fluorination of tryptophan indoles modifies dipole orientation and magnitude.
  • To determine the impact of these modifications on gramicidin channel ion conductance.

Main Methods:

  • Utilized deuterium ((2)H) solid-state NMR on aligned lipid bilayers.
  • Introduced fluorination (5- and 6-fluoro) to tryptophan indoles.
  • Analyzed (19)F-(2)H dipolar interactions for structural assignments.

Main Results:

Related Experiment Videos

  • Fluorination induced minor changes in side chain conformation.
  • High-resolution structures of fluorinated indoles at positions 11, 13, and 15 were determined.
  • Orientation and position of dipoles relative to the channel axis were measured.

Conclusions:

  • Modified dipoles' electrostatic interactions with cations can be predicted.
  • The influence of altered dipoles on ion conductance was estimated.
  • Direct measurements support the role of electrostatic interactions in channel permeation.