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Related Experiment Videos

Intracellular ice formation is affected by cell interactions.

J P Acker1, A Larese, H Yang

  • 1Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, T6G 2R8, Canada.

Cryobiology
|July 22, 1999
PubMed
Summary

Cell-cell and cell-surface interactions significantly influence intracellular ice formation in tissues. These interactions impact freezing temperatures and nucleation, crucial for understanding tissue response to cold.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Cryobiology

Background:

  • Cell-cell and cell-surface interactions are fundamental to tissue structure and function.
  • These interactions are critical determinants of how tissues respond to low temperatures.

Purpose of the Study:

  • To investigate the impact of cell-cell and cell-surface interactions on intracellular ice formation.
  • To analyze the influence of varying adhesion models on freezing kinetics in fibroblast cells.

Main Methods:

  • Utilized four in vitro models of hamster fibroblast cells in tissue culture.
  • Employed cryomicroscopy to observe intracellular ice formation after extracellular ice nucleation.

Main Results:

  • Both cell-cell and cell-surface interactions significantly altered the temperature of intracellular freezing.

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  • Evidence of intercellular nucleation, initiated through cell-cell contacts, was observed.
  • The prevalence and kinetics of intracellular ice formation were demonstrably affected by adhesion types.

    • Conclusions:

    • Cell-cell and cell-surface interactions play a substantial role in the low-temperature response of tissue systems.
    • Understanding these interactions is key to predicting and managing cellular damage during cryopreservation or cold exposure.