Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ploidy study in sporothrix schenkii

Torres-Guerrero1

  • 1Departmento de Microbiologia y Parasitologia, Facultad de Medicina UNAM, D.F., 04510, Mexico.

Fungal Genetics and Biology : FG & B
|July 22, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same journal

The phospholipid flippase DNF-4 is implicated in membrane trafficking and hyphal development in Neurospora crassa.

Fungal genetics and biology : FG & B·2026
Same journal

The sepD5 mutation of Aspergillus nidulans localizes to a gene encoding a LIM domain protein required for contractile ring constriction and recruitment of key septation proteins.

Fungal genetics and biology : FG & B·2026
Same journal

Functional analyses of secretory phospholipases A2 in Neurospora crassa.

Fungal genetics and biology : FG & B·2026
Same journal

Targeted deletion of cc63tf and cc12grf Reveals Opposing Regulation of beauvericin Biosynthesis in Cordyceps cicadae.

Fungal genetics and biology : FG & B·2026
Same journal

Identification of a regulatory factor activating the xylanase promoter of the basidiomycetous yeast Pseudozyma antarctica and its application to enhance endogenous biodegradable plastic-degrading enzyme production via transcription factor multiplexing.

Fungal genetics and biology : FG & B·2026
Same journal

A microfluidic spore chamber for long-term imaging of single-spore hyphal development.

Fungal genetics and biology : FG & B·2026
See all related articles

Sporothrix schenckii, a human pathogen, was investigated for its ploidy. Experiments suggest this dimorphic fungus is diploid, though aneuploidy remains a possibility.

Area of Science:

  • Mycology
  • Medical Mycology
  • Fungal Genetics

Background:

  • Sporothrix schenckii is a dimorphic fungus pathogenic to humans.
  • Its sexual cycle and genetic makeup are poorly understood.
  • Determining the ploidy state is crucial for understanding its genetics.

Purpose of the Study:

  • To investigate the ploidy of Sporothrix schenckii.
  • To analyze DNA content, UV resistance, and mutagenesis in S. schenckii.
  • To compare S. schenckii's genetic behavior to known fungal models.

Main Methods:

  • DNA content analysis during fungal phase transition.
  • Assessment of survival rates after ultraviolet (UV) irradiation.
  • Chemical mutagenesis (nitrous acid, UV light) to induce auxotrophy and albinism.

Related Experiment Videos

  • Induction of mitotic recombination.
  • Comparison with Saccharomyces cerevisiae (haploid and diploid).

    • Main Results:

    • No ploidy changes were observed between conidia and yeast phases.
    • S. schenckii exhibited higher UV resistance, similar to diploid Saccharomyces cerevisiae.
    • Mutagenesis induced albino colonies in one step and auxotrophic requirements (adenine, methionine) in two steps.
    • Revertants behaved as heterozygotes, yielding original mutations after UV exposure.

    Conclusions:

    • Experimental evidence strongly suggests Sporothrix schenckii is diploid.
    • Aneuploidy cannot be definitively ruled out.
    • These findings provide insights into the genetic constitution of this important human fungal pathogen.