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Cloning of insertion sequence IS1485 from Enterococcus species.

S Q Cheng1, R E Musso, R Liu

  • 1Institute of Molecular Biology and Medicine, University of Scranton, Scranton, Pennsylvania 18510, USA.

Plasmid
|July 22, 1999
PubMed
Summary

Researchers identified IS1485, a novel insertion sequence found in various Enterococcus species. This transposable element, resembling IS3 family members, was detected in multiple strains, including clinical isolates.

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Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Insertion sequences (IS) are mobile genetic elements that play a role in genome evolution.
  • The genus Enterococcus harbors diverse genetic elements, contributing to its adaptability.

Purpose of the Study:

  • To clone and characterize a novel insertion sequence, IS1485, identified in Enterococcus species.
  • To determine the distribution and genetic organization of IS1485 within the Enterococcus genus.

Main Methods:

  • Cloning and sequencing of the IS1485 element.
  • Bioinformatic analysis to determine genetic organization and identify open reading frames (ORFs).
  • Comparative sequence analysis to identify homologous elements.

Main Results:

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  • IS1485 was identified in multiple Enterococcus species, including E. durans, E. faecium, E. faecalis, E. avium, E. gallinarum, E. casseliflavus, and E. saccharolyticus.
  • The element IS1485 is 1366 bp long with imperfect terminal inverted repeats and contains three ORFs (orfA, orfB, orfC).
  • The genetic organization of IS1485 shows resemblance to the IS3 family of transposable elements, with sequence homology to IS199.

Conclusions:

  • IS1485 is a novel insertion sequence prevalent in the Enterococcus genus.
  • Its genetic structure suggests it belongs to the IS3 family of transposable elements.
  • IS1485 may contribute to the genetic diversity and evolution of Enterococcus species.