Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Studies on conditional gene expression in the brain.

J Jerecic1, F Single, U Krüth

  • 1Department of Molecular Neuroscience, Max-Planck Institute for Medical Research, Heidelberg, Germany.

Annals of the New York Academy of Sciences
|July 22, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ACU193, a Monoclonal Antibody that Selectively Binds Soluble Aß Oligomers: Development Rationale, Phase 1 Trial Design, and Clinical Development Plan.

The journal of prevention of Alzheimer's disease·2023
Same author

Posterior fossa syndrome with a large inflammatory ponto-mesencephalic lesion.

Brain and cognition·2016
Same author

Higher than expected estradiol levels in aromatase inhibitor-treated, postmenopausal breast cancer patients.

Climacteric : the journal of the International Menopause Society·2012
Same author

Assessment of early postpartum reproductive performance in two high producing Estonian dairy herds.

Acta veterinaria Scandinavica·2004
Same author

Impaired NMDA receptor function in mouse olfactory bulb neurons by tetracycline-sensitive NR1 (N598R) expression.

Brain research. Molecular brain research·2001
Same author

AMPA receptor channels with long-lasting desensitization in bipolar interneurons contribute to synaptic depression in a novel feedback circuit in layer 2/3 of rat neocortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2001

Researchers explored methods to control gene expression for postsynaptic machinery components. They developed novel genetic tools for precise regulation of target genes in vitro and in vivo.

Area of Science:

  • Molecular biology
  • Neuroscience
  • Genetics

Background:

  • Precise control over gene expression is crucial for understanding cellular functions, particularly in complex systems like the postsynaptic machinery.
  • Existing methods for gene regulation have limitations in specificity and applicability.

Purpose of the Study:

  • To develop and evaluate novel strategies for regulating gene expression in vitro and in vivo.
  • To investigate tetracycline-transactivator-dependent (tTA) regulation and Cre-lox recombination systems for gene manipulation.
  • To introduce a knockin approach for targeted, artificial regulation of endogenous genes.

Main Methods:

  • Utilized tTA-dependent gene expression systems.
  • Employed Cre recombinase in conjunction with reversible silencing via intron engineering of dominant-negative alleles.

Related Experiment Videos

  • Developed and tested a knockin strategy for site-specific gene regulation.

    • Main Results:

    • Demonstrated successful in vitro and in vivo regulation of genes encoding postsynaptic machinery components.
    • Validated the efficacy of tTA-dependent systems and Cre-mediated recombination for gene control.
    • Showcased the potential of the knockin approach for precise, on-site gene regulation.

    Conclusions:

    • Developed versatile genetic tools for precise control of gene expression relevant to postsynaptic function.
    • The presented methods offer improved strategies for studying gene function and developing therapeutic interventions.
    • The knockin approach provides a powerful platform for artificial regulation of chromosomal genes.