Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

R-cadherin expression in the developing and adult zebrafish visual system.

Q Liu1, K L Sanborn, N Cobb

  • 1Department of Anatomy and Cell Biology, 4610 Medical Science II, University of Michigan, Ann Arbor 48109, USA. qliu@umich.edu

The Journal of Comparative Neurology
|July 22, 1999
PubMed
Summary

Researchers identified a new R-cadherin gene in zebrafish, crucial for neural development. This cell adhesion molecule is vital for forming and maintaining neuronal connections in the zebrafish visual system.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Land Use, Not Stream Order, Controls N<sub>2</sub>O Concentration and Flux in the Upper Mara River Basin, Kenya.

Journal of geophysical research. Biogeosciences·2020
Same author

Cell adhesion molecule cadherin-6 function in zebrafish cranial and lateral line ganglia development.

Developmental dynamics : an official publication of the American Association of Anatomists·2011
Same author

Twelve testable hypotheses on the geobiology of weathering.

Geobiology·2011
Same author

Differential expression of photoreceptor-specific genes in the retina of a zebrafish cadherin2 mutant glass onion and zebrafish cadherin4 morphants.

Experimental eye research·2006
Same author

Cloning and expression analysis of cadherin7 in the central nervous system of the embryonic zebrafish.

Gene expression patterns : GEP·2006
Same author

Notch-Delta signaling is required for spatial patterning and Müller glia differentiation in the zebrafish retina.

Developmental biology·2005

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Molecular Biology

Background:

  • Cell adhesion molecules, particularly cadherins, are essential for tissue development and function.
  • Zebrafish are a key model organism for developmental studies, yet their cadherin repertoire is incompletely understood.

Purpose of the Study:

  • To systematically identify novel cadherin molecules in zebrafish.
  • To characterize the expression pattern of a newly identified zebrafish R-cadherin homolog.

Main Methods:

  • Screening of zebrafish cDNA libraries using an antibody against mouse E-cadherin.
  • Northern analysis, RNase protection assays, and in situ hybridization to determine R-cadherin expression.
  • Retrograde labeling of optic nerves to confirm R-cadherin expression in retinal ganglion cells.

Related Experiment Videos

Main Results:

  • A partial cDNA homologous to R-cadherin was identified in zebrafish.
  • Zebrafish R-cadherin expression increases during embryogenesis and persists in adults, particularly in the brain and retina.
  • Expression is localized to specific neuronal populations, including retinal ganglion cells, during critical periods of neural development.

Conclusions:

  • The identified zebrafish R-cadherin plays a role in the development and maintenance of neuronal connections within the visual system.
  • This finding expands the known cadherin family in zebrafish and highlights R-cadherin's importance in neural circuitry.