Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CpG oligodeoxynucleotides down-regulate macrophage class II MHC antigen processing.

R S Chu1, D Askew, E H Noss

  • 1Department of Pathology, Division of Infectious Diseases, Case Western Reserve University, Cleveland, OH 44106, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|July 22, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

When a patient's ethnicity is declared, medical students' decision-making processes are affected.

Internal medicine journal·2015
Same author

Some comments on male circumcision.

The Israel Medical Association journal : IMAJ·2013
Same author

Disordered Toll-like receptor 2 responses in the pathogenesis of pulmonary sarcoidosis.

Clinical and experimental immunology·2013
Same author

Strategies for assessing the degree of photodamage to skin: a systematic review of the literature.

The British journal of dermatology·2011
Same author

Immunostimulatory CpG motifs and DNA vaccines.

Methods in molecular medicine·2011
Same author

Commentary: male circumcision for prevention of heterosexual acquisition of HIV in men: perspective from a trial team.

International journal of epidemiology·2010
Same journal

Crystal structure and immune single-cell atlas provide insights into the functional divergence of type I IFNs in fish.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Complement C3 deficiency increases the effector and cytotoxic functions of NK cells and suppresses tumor growth.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

Increased Nur77 is disconnected from TCR affinity in insulin-specific Tregs.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

FTR85 negatively regulates type I IFN antiviral signaling pathway by promoting K48-linked polyubiquitination of IRF3.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

An MR1-specific nanobody capable of blocking MR1T cell activation.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same journal

TGF-β controls developmental fate and functional identity of thymic γδ T cells.

Journal of immunology (Baltimore, Md. : 1950)·2026
See all related articles

CpG oligodeoxynucleotides (ODN) activate immune cells but inhibit macrophage antigen processing and presentation. This effect is linked to reduced major histocompatibility complex class II (MHC-II) molecule synthesis after prolonged exposure.

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • Unmethylated CpG motifs in DNA stimulate immune cells, acting as adjuvants.
  • CpG DNA activates macrophages, but its impact on antigen processing is unclear.

Purpose of the Study:

  • To investigate the effects of CpG oligodeoxynucleotides (ODN) on antigen (Ag) processing and presentation by macrophages.
  • To determine the impact of CpG ODN on major histocompatibility complex class II (MHC-II) expression and related gene synthesis.

Main Methods:

  • Peritoneal macrophages were cultured with CpG ODN and pulsed with protein antigens.
  • Antigen presentation was assessed using T cell hybridomas.
  • MHC-II expression and mRNA levels were analyzed via flow cytometry and Northern blot, respectively.

Related Experiment Videos

Main Results:

  • CpG ODN and LPS inhibited the processing of bovine RNase and hen egg lysozyme.
  • Prolonged (18 h) CpG ODN treatment decreased surface MHC-II expression and I-Ak mRNA levels.
  • Endocytosis was unaffected, and inhibition was observed only after extended CpG ODN exposure.

Conclusions:

  • CpG ODN exposure leads to decreased macrophage antigen processing and presentation.
  • The primary mechanism involves a reduction in MHC-II molecule synthesis.
  • Unlike some studies, CpG ODN did not enhance macrophage antigen processing.