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Prolactin receptor localization to the area postrema.

L P Mangurian¹, A R Jurjus, R J Walsh

¹347 Smith Hall, Department of Biological Sciences, Towson University, Towson, MD 21252-0001, USA. lmangurian@towson.edu

|July 23, 1999

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Researchers found specific prolactin binding sites in the area postrema, a brain region without a blood-brain barrier. This indicates the area postrema is a novel target for prolactin in the central nervous system (CNS).

Area of Science:

Neuroendocrinology
Neuroscience
Receptor Binding Studies

Background:

The area postrema lacks a blood-brain barrier, suggesting it could be a site for direct vascular prolactin interaction with neurons.
Prolactin's role in the central nervous system (CNS) is not fully understood, particularly in areas lacking a protective barrier.

Purpose of the Study:

To investigate the presence and specificity of prolactin receptors in the area postrema.
To determine if the area postrema is a potential target for circulating prolactin.

Main Methods:

In vitro autoradiography was used on frozen sections of rabbit medulla.
Sections were incubated with radiolabelled ovine prolactin to assess total binding.
Non-specific binding was determined using excess unlabelled ovine prolactin, human prolactin, or ovine luteinizing hormone (LH).

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Main Results:

Specific prolactin binding sites were identified within the area postrema.
Excess unlabelled ovine and human prolactin significantly reduced radiolabelled prolactin binding.
Unlabelled ovine LH did not affect radiolabelled prolactin binding, indicating specificity.

Conclusions:

The area postrema possesses specific prolactin binding sites.
This finding identifies the area postrema as a previously unrecognized target area for prolactin within the CNS.
Vascular prolactin may directly influence neuronal elements in the area postrema.