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Study of MFD in Bacillus subtilis.

V D Filippov, E E Zagoruiko

    Mutation Research
    |October 1, 1978
    PubMed
    Summary

    UV radiation can decrease mutation frequency in bacteria under specific conditions, suggesting an anti-mutagenic DNA repair mechanism. This mutation frequency decline (MFD) is linked to excision repair activity and influenced by nutrient availability and protein synthesis.

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    [Photoreactivity of premutation damage caused by UV irradiation at the suppressor locus of Escherichia coli chromosomes].

    Doklady Akademii nauk SSSR·1991

    Area of Science:

    • Microbiology
    • Molecular Biology
    • Genetics

    Background:

    • UV radiation induces DNA damage, potentially leading to mutations.
    • Bacterial DNA repair mechanisms, such as excision repair, counteract mutagenesis.
    • Extragenic suppressor reversions provide a model to study mutation frequency changes.

    Purpose of the Study:

    • To investigate the phenomenon of mutation frequency decline (MFD) in UV-irradiated *B. subtilis*.
    • To elucidate the role of excision repair in MFD.
    • To determine the influence of pre-irradiation conditions and post-irradiation treatments on MFD.

    Main Methods:

    • Utilizing *B. subtilis* strains with a *leu8* mutation.
    • Irradiating cells with UV light and observing reversion frequency to leucine independence.
    • Manipulating incubation conditions (nitrogen deprivation, nutrient availability) and chemical treatments (acriflavine).
    • Assessing MFD in wild-type (*uvr+*) and mutant (*uvr1*) strains.

    Main Results:

    • MFD was observed when UV-irradiated cells were incubated in nitrogen-deprived medium.
    • Acriflavine inhibited MFD, and it was reduced in *uvr1* mutants, indicating a role for excision repair.
    • Prolonged nitrogen starvation before irradiation reduced MFD, while post-irradiation incubation in nutrient medium accelerated it.
    • MFD stimulation by UV was dependent on protein synthesis and observed only in the *uvr+* strain.

    Conclusions:

    • MFD is proposed to be a manifestation of the anti-mutagenic activity of excision repair.
    • The rate of MFD is influenced by the cell's physiological state and pre-radiation history, reflecting varying levels of excision-repair activity.
    • Excision repair plays a crucial role in reducing UV-induced mutation frequency under specific cellular conditions.

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