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Updated: Aug 16, 2026

Detection of Histone Modifications in Plant Leaves
Published on: September 23, 2011
Transcriptional activation by Arabidopsis GT-1 may be through interaction with TFIIA-TBP-TATA complex
J Le Gourrierec1, Y F Li, D X Zhou
1Laboratoire de Génétique Moléculaire des Plantes, Université de Picardie Jules Verne, Amiens, France.
Abstract:
GT-1 belongs to the class of trihelix DNA-binding proteins and binds to a promoter sequence found in many different genes. Data presented in this report show that GT-1 contains a trans-activation function in yeast and in plant cells. However, in tobacco BY-2 protoplasts, this activity functions only when an internal region containing the DNA-binding domain is deleted. Gel-shift and co-immunoprecipitation assays have revealed that GT-1 can interact with and stabilize the TFIIA-TBP-TATA complex. These results suggest that GT-1 may activate transcription through direct inter- action with the transcriptional pre-initiation complex.
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