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Characterization of bovine coronavirus isolates/from eight different states in the USA
S Kapil1, K L Richardson, T R Maag
1Department of Diagnostic Medicine-Pathobiology College of Veterinary Medicine, Kansas State University, Manhattan 66506, USA. kapil@vet.ksu.edu
Abstract:
Bovine coronavirus isolates from eight different states of the USA were compared for their antigenic properties and susceptibility to hygromycin B. Antigenic differences were observed among the isolates in a one-way hemagglutination-inhibition (HI) test using a polyclonal antiserum against the Mebus bovine coronavirus isolate. Differences were observed on isoelectric focusing among viral proteins with isoelectric points between 4.45-4.65. Most of the BCV isolates were susceptible to hygromycin B (0.5 mM) whereas a few hygromycin B resistant isolates were also found.