Related Experiment Video

Updated: Oct 19, 2026

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery
10:03

Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal Delivery

Published on: November 5, 2019

Immunogenicity of various presentation forms of PorA outer membrane protein of Neisseria meningitidis in mice

C C Peeters1, I J Claassen, M Schuller

  • 1Laboratory for Vaccine development and Immune Mechanisms, National Institute of Public Health and Environment, Bilthoven, The Netherlands.

Vaccine
|July 27, 1999
PubMed

Insights

Outer membrane vesicles (OMV) and immune-stimulating complexes (iscom) containing meningococcal PorA protein effectively generate bactericidal antibodies. Purified PorA, however, did not elicit a significant response, highlighting the importance of formulation for vaccine efficacy.

Area of Science:

  • Immunology
  • Vaccinology
  • Microbiology

Background:

  • Neisseria meningitidis poses a significant public health threat.
  • The PorA outer membrane protein is a key target for meningococcal vaccines.
  • Evaluating different vaccine formulations is crucial for optimizing immunogenicity.

Purpose of the Study:

  • To compare the immunogenicity of various meningococcal PorA vaccine formulations.
  • To assess the ability of different formulations to induce bactericidal antibodies.
  • To analyze the antibody subclass responses elicited by each formulation.

Main Methods:

  • Comparison of purified PorA, outer membrane vesicles (OMV), and immune-stimulating complexes (iscom) formulations.
  • Assessment of antibody production, including bactericidal activity and OMV-binding antibodies.
  • Analysis of IgG subclass responses (IgG1, IgG2a, IgG2b, IgG3).

Main Results:

  • OMV and iscom formulations induced bactericidal antibodies, unlike purified PorA.
  • Both OMV and iscom formulations showed similar immunogenicity in dose-response studies.
  • PorA in OMV formulation induced a broad IgG subclass response, while iscoms favored IgG1 and antibodies against linear peptides.

Conclusions:

  • Meningococcal PorA outer membrane vesicles (OMV) and immune-stimulating complexes (iscoms) are effective vaccine formulations for generating bactericidal antibodies.
  • Only iscoms and OMV formulations, not purified PorA, elicit a robust immune response, including anamnestic and bactericidal antibody production.
  • Formulation is critical for the immunogenic potential of PorA-based meningococcal vaccines.

More Related Videos

Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development12:53

Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

Published on: March 16, 2022

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice08:52

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

Published on: February 22, 2019

Related Concept Videos

Bacterial Meningitis II: Pathophysiology01:26

Bacterial Meningitis II: Pathophysiology

Bacterial meningitis typically begins when pathogens such as Neisseria meningitidis and Streptococcus pneumoniae colonize the nasopharynx and invade the bloodstream. This process is facilitated by bacterial virulence factors, such as polysaccharide capsules, which resist phagocytosis and complement-mediated killing. Less commonly, bacteria reach the central nervous system via contiguous spread from infections like otitis media or sinusitis, through congenital or acquired dural defects, or...
Bacterial Meningitis01:24

Bacterial Meningitis

Bacterial meningitis is a severe infectious disease involving inflammation of the meninges, the protective membranes surrounding the brain and spinal cord. It occurs when pathogenic bacteria cross the blood–brain barrier and enter the cerebrospinal fluid. Common causative organisms include Neisseria meningitidis, Streptococcus pneumoniae, Haemophilus influenzae type b, Listeria monocytogenes, and Escherichia coli K1. The exact route of entry varies by pathogen and host condition.Routes of Entry...